production of monoclonal antibody against human nestin
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abstract
we have employed a peptide-based antibody generation protocol for producing antibody against human nestin. using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any n- or o-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. a wide variety of nestin proteins ranging from 140-250 kda was detected by this antibody. this antibody is highly specific and functional in applications such as elisa, flow cytometry, immunocytochemistry, and western blot assays.
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Journal title:
avicenna journal of medical biotechnologyجلد ۲، شماره ۲، صفحات ۶۹-۷۷
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